IMMUNOMODULATORY, ANTICANCER AND ANTIOXIDANT ACTIVI TIES OF CYCLEA PELTATA (LAM.) HOOK. F. AND THOMSON
By: Sony, Jayaraman
.
Contributor(s): Variyar, E. Jayadevi.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(10).Description: 40-46p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
The present study was performed to evaluate the imm
unomodulatory, anticancer, and antioxidative proper
ties of the fraction (CP_2)
isolated from
Cyclea peltata
.
Methods:
Immunomodulation was evaluated in lymphocytes by ly
mphocyte proliferation assay and in THP-1 macrophag
e cell lines by MTT assay.
The nitrite production by the macrophages was also
measured by the nitrite assay using griess reagent.
The anticancer activity of the fraction was
determined by MTT assay. The antioxidant activity w
as evaluated by DPPH assay and total antioxidant ass
ay by phosphomolybdenum method. It is
expressed as number of gram equivalent of ascorbic
acid.
Results:
The plant fraction showed the presence of flavonoid
s which induced lymphocyte proliferation rate of 4.
29±0.007 at 100 μg/ml. It was not
toxic to THP-1 macrophage cells and also could induc
e nitrite production at 1 mg/ml. It also exhibited
good anticancer activity at 100 μg/ml after
48h of incubation. The DPPH activity was found to be
low since 100 μg/ml showed only an inhibition rate
of 22±0.026. The total antioxidant activity
at 1000 μg/ml of CP_2 was found to be equivalent to
79±0.03 μg/ml of ascorbic acid exhibiting moderate
antioxidant activity.
Conclusion:
The fraction CP_2 containing flavonoid, isolated fr
om
Cyclea peltata
has good immunomodulatory, antioxidant and antican
cerous property.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020863 |
Objective:
The present study was performed to evaluate the imm
unomodulatory, anticancer, and antioxidative proper
ties of the fraction (CP_2)
isolated from
Cyclea peltata
.
Methods:
Immunomodulation was evaluated in lymphocytes by ly
mphocyte proliferation assay and in THP-1 macrophag
e cell lines by MTT assay.
The nitrite production by the macrophages was also
measured by the nitrite assay using griess reagent.
The anticancer activity of the fraction was
determined by MTT assay. The antioxidant activity w
as evaluated by DPPH assay and total antioxidant ass
ay by phosphomolybdenum method. It is
expressed as number of gram equivalent of ascorbic
acid.
Results:
The plant fraction showed the presence of flavonoid
s which induced lymphocyte proliferation rate of 4.
29±0.007 at 100 μg/ml. It was not
toxic to THP-1 macrophage cells and also could induc
e nitrite production at 1 mg/ml. It also exhibited
good anticancer activity at 100 μg/ml after
48h of incubation. The DPPH activity was found to be
low since 100 μg/ml showed only an inhibition rate
of 22±0.026. The total antioxidant activity
at 1000 μg/ml of CP_2 was found to be equivalent to
79±0.03 μg/ml of ascorbic acid exhibiting moderate
antioxidant activity.
Conclusion:
The fraction CP_2 containing flavonoid, isolated fr
om
Cyclea peltata
has good immunomodulatory, antioxidant and antican
cerous property.
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